四川大学生命科学学院研究生导师：张义正

　　姓名：张义正　性别：男

　　职称：教授　学院：生命科学学院

　　联系方式：028-85412738(电话和传真)

　　Email：yizzhang@scu.edu.cn

　　学位简历和工作简历：

　　1965.09-1970.06就读于四川大学生物学系，

　　1982.03-1987.12就读于美国密西根州立大学微生物学系，获博士学位。

　　1970.07-1982.02于四川大学生物学系任教，主要从事微生物学教学和科研工作;

　　1988.01-至今在四川大学生命科学学院从事本科生、研究生教学工作和指导研究生，以及分子生物学和生物技术领域的科研工作。

　　研究方向及研究工作

　　1)研究方向

　　分子遗传学及基因工程;基因组学。

　　2)目前研究工作重点

　　木质纤维素降解真菌黄孢原毛平革菌(Phanerochaete chrysosporium)和粗毛栓菌(Trametes gallica)的转录组分析及其木质纤维素降解酶类基因的克隆、表达和调控机制的研究;运动发酵单胞菌(Zymomonas mobilis)比较基因组学分析和基因工程菌建立;大熊猫肠道抗生素抗性细菌基因组分析及其抗性相关基因的研究;非粮高淀粉作物甘薯(Ipomoea batatas (L). Lam)的转录组和数字表达谱分析，以及影响淀粉产量相关基因的克隆、表达和应用研究。

　　3)取得的主要研究进展

　　构建了甘薯不同组织和块根不同发育阶段的转录组序列、基因注释和数字表达谱数据库，克隆了多个与淀粉合成、抗逆、抗病、开花和转座相关基因以及它们在不同组织或发育阶段的表达规律。分别构建了黄孢原毛平革菌和粗毛栓菌在多种培养条件下的转录组序列和基因注释数据库，在粗毛栓菌转录组中已发现多个木质纤维素降解酶基因，如锰过氧化物酶、漆酶、木质素过氧化物酶等基因。构建了运动发酵单胞菌(Zymonomyces mobilis)限制修饰系统缺陷型菌株和可利用甘薯淀粉进行乙醇发酵的基因工程菌，完成了3株运动发酵单胞菌的基因组测序、基因注释和比较基因组研究。完成了2株大熊猫肠道抗生素高抗细菌的基因组测序和组装，其中1株大肠杆菌菌株中含有多个高拷贝质粒，这些质粒与细菌的抗生素高抗特性相关。

　　近期发表和拟发表文章

　　Wang GJ, Wang ZS,Zhang YW,Zhang YZ.Cloning and expression of amyE gene from Bacillus subtilis in Zymomonas mobilis and direct production of ethanol from soluble starch. Biotechnology and Bioprocess Engineering (accepted).2012

　　Chang MJ, Tao X, Gu YH, Shao HH, Cao QH, Zhang YZ. Cloning and characterization of the 14-3-3 protein gene from Ipomoea batatas (L.) Lam. African Journal of Microbiology Research (accepted). 2012

　　Tao X, Gu YH, Wang HY, Zheng W, Li X, Zhao CW, Zhang YZ. Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.]. PLosOne (under review). 2012

　　Jiang YS, Chen C, Tao X，Zhang YZ. A proteomic analysis of storage stress responses in Ipomoea batatas (L.) Lam. tuberous root. Molecular Biology Reports (under review). 2012

　　Qin H, Gu Q, Zhang JL, Sun L, Kuppu S, Zhang YZ, Burow M, Payton P, Blumwald E, Zhang H. Regulated Expression of an Isopentenyltransferase Gene (IPT) in Peanut Significantly Improves Drought Tolerance and Increases Yield Under Field Conditions. Plant and Cell Physiology. 52 (11): 1904-1914. 2011

　　Wang ZS, Xiang QJ, Wang GJ, Wang HY and Zhang YZ. Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion. Genetics and Molecular Biology. 34(4):661-668. 2011.

　　Liu Z, Li X, Zhao P, Gui JH, Zheng W, Zhang YZ. 2011. Tracing the evolution of the mitochondrial protein import machinery. Computational Biology and Chemistry. 35:336–340. 2011

　　Li Y, Gu YH, Qin H, Zhang YZ. Two pairs of sucrose transporters in Ipomoea batatas (L.) Lam are predominantly expressed in sink leaves and source leaves respectively. Plant Science. 179:250–256. 2010

　　Xu K, He BW, Zhou S, Li Y, Zhang YZ. Cloning and characterization of the Rubisco activase gene from Ipomoea batatas (L.) Lam. Mol Biol Rep 37:661–668. 2010

　　Wu JM, Zhang YZ. Gene Expression in Secondary Metabolism and Metabolic Switching Phase of Phanerochaete chrysosporium. Appl Biochem Biotechnol. 162(7):1961-77. 2010

　　Jiang MF, Li X, Zhang L, Feng H, Zhang YZ. Gene expression analysis of Phanerochaete chrysosporium during the transition time from primary growth to secondary metabolism. The Journal of Microbiology. 47(3):308-318. 2009

　　Fan ZX, Yue CW, Tang Y, Zhang YZ. Cloning, sequence analysis and expression of bacterial lipase-coding DNA fragments from environment in Escherichia coli. Mol Biol Rep. 36:1515–1519. 2009

　　He MX, Li Y, Liu X, Bai F, Feng H, Zhang YZ. Ethanol production by mixed-cultures of Paenibacillus sp. and Zymomonas mobilis using the raw starchy material from sweet potato. Annals of Microbiology. 59 (4) :749-754. 2009

　　He MX, Feng H, Bai F, Li Y, Liu X, Zhang YZ. Direct production of ethanol from raw sweet potato starch using genetically engineered Zymomonas mobilis. African Journal of Microbiology Research. 3(11):721-726. 2009

　　Yue CW, Zhang YZ. Cloning and expression of Tenebrio molitor antifreeze protein in Escherichia coli. Mol Biol Rep. 36:529–536. 2009

　　Tang Y, Tan XM, Yue CW, Li CX, Fan ZX, Zhang YZ. Cloning, Sequence, and Function Analyses of Giant Panda (Ailuropoda melanoleuca) CD9 Gene. Mol. Reprod Dev. 75(9):1418-1425. 2008

　　Bai F, Zeng CM, Yang SX, Zhang YZ, He Y, Jin J. The formation of a novel supramolecular structure by amyloid of poly-L-glutamic acid. Biochem. Biophys. Res. Commun. 369:830–834. 2008

　　He MX, Feng H, Zhang YZ. Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif. Biotechnol. Lett. 30(12):2111-2117. 2008

　　He FM, Zhu YP, Zhang YZ. Identification and characterization of differentially expressed genes involved in pharmacological activities of roots of Panax notoginseng during plant growth. Plant Cell Rep. 27(5):923-930. 2008

　　He FM, Zhu YP, He MX, Zhang YZ. Molecular cloning and characterization of the gene encoding squalene epoxidase in Panax notoginseng. Mitochondria DNA. 19(3):270-273. 2008.

　　授权发明专利

　　张义正、刘德明、刘彦、程昌凤、黄庆. 脱毛蛋白酶、其制造方法、使用方法和产生此蛋白酶的微生物.ZL00120641.9

　　张义正、李珉、王海燕、童英. 脱毛蛋白酶和编码这种脱毛蛋白酶的多核苷酸. ZL03135279.0