四川大学生命科学学院研究生导师：梁朋

　　姓名：梁朋　性别：男

　　职称：教授　学院：生命科学学院

　　简历：

　　北京大学生物系学士，美国伊利诺斯大学生物化学博士，美国哈佛大学医学院 Dana-Farber癌症研究所博士后，美国 Vanderbilt大学终身教授。是基因差异显示技术和蛋白质三聚体化技术的发明人、白介素24(IL-24)及受体的发现者、p53靶基因 Killin,NDRG1,CYFIP2和TIS11D 的发现者,拥有10项已授权的美国专利。1997年获北美华人生物学家年会(SCBA)颁发的“科技发明大奖”, 1998年获德国分子生物学和生物化学协会颁发的“分子分析大奖”，2009年中组部“千人计划”入选者。

　　职务：

　　美国Vanderbilt(范德堡)大学 终身教授 (Associate professor)

　　GenHunter公司 (美国) 创始人及董事长

　　四川三叶草生物制药公司 创始人及董事长

　　四川大学生命科学学院 教授

　　成都市第四届科技顾问团 顾问

　　电话：028-85336968

　　email：liangpeng@scu.edu.cn

　　简历：

　　北京大学生物系 学士

　　CUSBEA中美交换计划 留美学生

　　美国伊利诺斯大学生物化学 博士

　　美国哈佛大学医学院 Dana-Farber癌症研究所 博士后

　　美国Vanderbilt(范德堡)大学助理教授(Assistant Professsor)

　　美国Vanderbilt(范德堡)大学终身教授 (Associate Professor)

　　1992.10-至今 GenHunter公司 (美国) 创始人及董事长

　　2007.6-至今 四川三叶草生物制药公司 创始人及董事长

　　2007.12-至今 国家生命科学与技术人才培养基地(四川三叶草生物制药有限公司与四川大学生命科学学院共建)主任

　　2007.9-2010.7 四川大学 客座教授

　　2007.9-2010.7 四川大学生命科学学院兼职教授

　　2008.5-至今 成都市第四届科技顾问团 顾问

　　2010.8-至今 四川大学 教授

　　研究方向：

　　癌症及自身免疫性疾病(如牛皮癣及关节炎)等的发病分子基理及治疗手段。

　　研究内容：

　　本实验室以运用世界上最先进的荧光基因表达差异显示(FDD)技术为手段，寻找及发现疾病相关的靶基因，用生物化学及细胞生物学的方法对其进行功能性研究。用AP-Tag技术寻找及研究相关的分泌型细胞因子及其细胞表面受体，并运用现代生物逻辑制药的方法(包括本实验室发明的蛋白质三聚体化技术)，在生物反应器中采用无血清细胞培养技术研发生产新一代癌症及自身免疫性疾病的靶向抗体及可溶性受体蛋白质药物。

　　从事科研项目：

　　(1)国家973项目《肿瘤发生发展中关键蛋白的功能与调控》的课题2《研究抑癌蛋白p53家族及新的上游调控蛋白和下游作用靶点对肿瘤发生发展的分子调控机制》

　　(2)国家自然科学基金项目《Killin在p53介导肿瘤抑制上的生物学功能》

　　发表论文：

　　在《科学》、《自然生物技术》、《自然方法学》、《自然癌症综述》、《美国国家科学院院士通报》和《酶学方法》等世界著名杂志上发表的文献近70篇，其ISI引用率超过9000次。

　　(1) Cho YJ, Liang P. S-phase-coupled apoptosis in tumor suppression. Cell Mol Life Sci. 2011 Jun;68(11):1883-96.

　　(2) He M, Liang P. IL-24 transgenic mice: in vivo evidence of overlapping functions for IL-20, IL-22, and IL-24 in the epidermis. J Immunol. 2010 Feb 15;184(4):1793-8.

　　(3) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Linear dynamic range for signal detection in fluorescent differential display. Biotechnol Lett. 2010 Nov;32(11):1593-7.

　　(4) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Proof-reading signal accuracy of gene expression by binary differential display. Biotechnol Lett. 2010 Aug;32(8):1039-44.

　　(5) Meade JD, Cho YJ, Shester BR, Walden JC, Guo Z, Liang P. Automated fluorescent differential display for cancer gene profiling. Methods Mol Biol. 2010;576:99-133.

　　(6) S. Zhou, J.D. Meade, S. Nathashon, B. Shester, J. Walden, Z. Guo, J.Z. Liang, J. G. Liang and P. Liang.: Genome-Wide Analysis of mRNA Expression by Florescent Differential Display. The Handbook of Plant Functional Genomics. p165-186. 2008. Wiley-Blackwell publisher.

　　(7) Cho YJ, Liang P. Killin is a p53-regulated nuclear inhibitor of DNA synthesis. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5396-401.

　　(8) Jackson, R., Cho YJ., Stein, S. and Liang, P.: CYFIP2 is a p53 target gene. Cell Cycle. 2007 Jan;6(1):95-103.

　　(9) Liang, P. Meade, J. and Pardee, A.B: "A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling" - Nature Protocols. 2007, Mar 15, 2:457 - 470.

　　(10) Liang, P. : MAQC papers over the cracks. Nature Biotechnology. 2007 Jan; 25(1): 27-8.

　　(11) Jackson, R., Cho YJ., and Liang, P.: TIS11D is a Candidate Pro-Apoptotic p53 Target Gene. Cell Cycle. 2006 Dec;5(24):2889-2893.

　　(12) Liang P.: From differential display to DNA microarrays-a personal account. J Cell Physiol. 2006 Dec;209(3):653-8.

　　(13) Tan Z, Wang M, Liang P.: Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes. Methods Mol Biol. 2005;317:207-18.

　　(14) Meade JD, Cho YJ, Fisher JS, Walden JC, Guo Z, Liang P.: Automation of fluorescent differential display with digital readout. Methods Mol Biol. 2005;317:23-58.

　　(15) Cho YJ, Stein S, Jackson RS, Liang P.: Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display. Methods Mol Biol. 2005;317:179-92.

　　(16) Yang S, Liang P.: Global analysis of gene expression by differential display: a mathematical model. Methods Mol Biol. 2005;317:3-22.

　　(17) Thomas EK, Nakamura M, Wienke D, Isacke CM, Pozzi A, Liang P.: Endo180 binds to the C-terminal region of alpha 1(I) collagen. (2005). J Biol Chem. 280(24):22596-605.

　　(18) Wang M, Liang P. Interleukin-24 and its receptors. (2005). Immunology. 114(2):166-70.

　　(19) Yang, S. and Liang P.: Global analysis of gene expression by differential display, a mathematical model. (2004). Mol Biotechnol. 3:197-208.

　　(20) Wang, M., Tan,Z., Thomas, E.K. and Liang, P.: Conservation of the genomic structure and receptor-mediated signaling between human and rat IL-24. (2004). Genes & Immunity. 5:363-70.

　　(21) Stein,S., Thomas, E.K., Herzog, B., Westfall, M. D., Rocheleau, J.V., Jackson, R., Wang, M. and Liang, P.: NDRG1 is necessary for p53-dependent apoptosis. (2004). J. Biol. Chem., 279(47):48930-40

　　(22) Peng Liang & Arthur B. Pardee.: Analysing differential gene expression in cancer. Nature Reviews Cancer (2003) 3:869-876.

　　(23) Stein S, Cho, Y. Jackson R.S. and Liang P.: Identification of p53 target genes by fluorescent differential display. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.

　　(24) Stein S, Liang P.: Differential display analysis of gene expression in mammals: a p53 story. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.

　　(25) Stein J, Liang P.: Differential display technology: a general guide. Cell Mol Life Sci. 2002 Aug;59(8):1235-40. Review.

　　(26) Liang P.: SAGE Genie: A suite with panoramic view of gene expression. Proc. Natl. Acad. Sci. U. S. A. (2002) 99:11547-11548.

　　(27) Liang P.: A decade of differential display. Biotechniques. (2002) 33:338-346.

　　(28) Cho, Y., Prezioso, V. R. And Liang, P.: Systematic analysis of intrisince factors affecting differential display. Biotechniques (2002), 32:1-4.

　　(29) Wang, M.,Tan,Z., Zhang, R.,Kotenko, S. V. and Liang, P.: Interleukin-24 (Mob-5/ Mda-7) Signals through Two Heterodimeric Receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. (2002) J. Biol. Chem. 277:7341-7347.

　　(30) Cho, Y., Meade, J., Walden, J., Guo, Z. and Liang, P.: Multi-color Fluorescent Differential Display. Biotechniques (2001), 30:562-572.

　　(31) Jo, H., Cho, Y., Zhang, H. and Liang P.: Differential display analysis of altered gene expression by ras oncogene. (2001) Methods in Enzymol. (Academic Press), 332: 233-244.

　　(32) Liang, P.: Gene Discovery Using Differential Display. Genetic Engineering News. (2000) 20:37.

　　(33) Cho, Y. and Liang, P.: Differential display for detecting potential gene candidates. Molecular Genetics of Mental Disorders, Edited by Briley and Sulser. Martin Dunitz Publishers, London, UK., (2000). In press.

　　(34) Zhang, R., Tan, Z. and Liang, P.: Identification of a novel ligand-receptor pair constitutively activated by ras oncogene. (2000) J. Biol. Chem. 275:24436-24443.

　　(35) Jo, H., Zhang, R., Zhang, H. McKinsey, T. A. Ballard, D.W. and Liang, P.: NF-kB is required for H-Ras oncogene induced abnormal cell proliferation and tumorigenesis. (2000), Oncogene 19:841-849.

　　(36) Liang, P. and Pardee, A. B.: Differential Display, a general Protocol. Mol. Biotechnology. (1998), 10:261-267.

　　(37) Jo, Hakryul, Zhang, H., Zhang, R. and Liang, P.: Cloning oncogenic Ras regulated genes by differential display. Methods ----- A Companion to Methods in Enzymology, Academic Press, (1998), Academic Press,16:365-375.

　　(38) Liang, P.: Factors Ensuring successful use of differential display. Methods ----- A Companion to Methods in Enzymology, Academic Press, (1998), Academic Press, 16:361-364.

　　(39) Sheng, H., Williams, C. S., Shao, J., Liang, P., DuBois, R. N. and Beauchamp, R. D.: Induction of cyclooxygenase-2 by activated Ha-ras oncogene in Rat-1 fibroblasts and the role of mitogen-activated protein kinase pathway. (1998) J. Biol. Chem. 273: 22120-22127.

　　(40) Zha, ng, R., Averboukh, L., Zhu, W., Zhang, H., Jo, H., Dempsey, P.J., Coffey, R., A. B. Pardee and Liang, P.: Identification of rCop-1, a New Member of CCN Gene Family, as a Negative Regulator for Cell Transformation. (1998), Mol. Cell. Biol., 18:6131-6141.

　　(41) Hu, J., You, S., Li, W., Wang, D., Nagpal, M. L., Mi, Y., Liang, P. and Lin, Tu.: Expression and regulation of Interferon-g-inducible protein 10 gene in Rat Leydig Cells. (1998) Endocrinology 139:3637-3645.

　　(42) Liang, P., Wang, F., Zhu, W., O'Connell, R. P., and Averboukh, L.: Identification of novel diagnostic markers by differential display. (1998) Methods in Molecular Medicine. Humana Press, p3-13.

　　(43) Grady, T., Liang, P., Ernst, S.A. and Logsdon, C. D.: Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis. (1997) Gastroenterology 113:1966-1975.

　　(44) Liang, P. and Pardee, A.B.: Differential display: A general protocol. Methods in Molecular Biology. (1997) Vol. 85, p3-12. Humana Press.

　　(45) Zhang, R., Zhang, H., Zhu, W., Coffey, R., A. B. Pardee and Liang, P.: Mob-1, a ras target gene, is over expressed in colorectal cancer. (1997) Oncogene 14:1607-1610.

　　(46) Averboukh, L., Liang, P., Kantoff, P. W. and Pardee, A.B.: Regulation of S100P expression by androgen. (1996) Prostate, 29:350-355.

　　(47) Zhu, W. and Liang, P.: Detection and isolation of differentially expressed genes by differential display. (1996) Methods in Molecular Biology, 68:211-220, Boultwood, J. ed. Humana Press

　　(48) Liang, P.: Analysis of messenger RNA by differential display. (1996) A laboratory guide to RNA, 223-236. Krieg, P.A. ed., Wiley-Liss.

　　(49) , Wang, F-L., Wang, Y., Wong, W-K., Liu, Y., Addivinola, F. J., Liang, P., Chen, L-B., Kantoff, P. W. and Pardee, A.B.: Two differentially expressed genes in normal human prostate tissue and in carcinoma. (1996) Cancer Res. 56:3634-3637.

　　(50) Zhang, H., Zhang, R. and Liang, P.: Differential screening of gene expression difference enriched by differential display. (1996) Nucleic Acids Res. , 24:2454-2456.

　　(51) Hu, E., Liang, P. and Spiegelman, B. M.: AdipoQ is a novel adipose-specific gene dysregulated in obesity. (1996) J. Biol. Chem. 271:10697-10703.

　　(52) Liang, P., Averboukh, L., Zhu, W., Wang, F., Haley, T. and Pardee, A. B.: Molecular characterization of Murine thymidylate kinase gene. (1995) Cell Growth & Differentiation 6:1333-1338.

　　(53) Trentmann, S.M., van der Knaap, E., Kende, H., Liang, P. and Pardee A.B.: Alternatives to 35S as a Label for the differential display of eukaryotic messenger RNA. (1995) Science 267: 1186-1187.

　　(54) Rohrwild, M., Alpan, R.S., Liang, P. and Pardee, A.B.: Inosine-containing primers for mRNA differential display. (1995) Trend in Genet., 11:300.

　　(55) Liang, P. and Pardee, AB.: Recent Advances in Differential Display. (1995) Current Opinion in Immunol. 7:274-280.

　　(56) Liang, P., Bauer, D., Averboukh, L., Warthoe, P. Rohrwild, M., Muller, H., Strauss, M. and Pardee, A.B.: Analysis of altered gene expression by differential display. (1995) in Oncogene Techniques, Methods in Enzymol. (Academic Press), 254:304-321.

　　(57) Liang, P., Zhu, W., Zhang, X., Guo, Z., O’Connell, R., Averboukh, L. Wang, F. and Pardee, A. B.: Differential display using one-base anchored oligo-dT primer. (1994) Nucleic Acids Res., 22:5763-5764.

　　(58) Liang, P. and Pardee, A.B., : Differ, ential display of mRNA by PCR. (1994) in Current Protocols in Molecular Biology (Wiley Interscience), Unit 15.8.

　　(59) Liang, P., Averboukh, L. and Pardee, A. B.: Method of differential display. (1994) in Methods in Molecular Genetics (Academic Press), Vol. 5, pp3-16.

　　(60) Liang, P., Averboukh, L., Zhu, W. and Pardee, A. B.: Ras activation of novel genes: Mob-1 as a model. (1994) Proc. Natl. Acad. Sci. U. S. A. , 91:12515-12519.

　　(61) Barry M. B., Meador, B., Builerback, T., Liang, P., Glaser, M. and Philips, G. N., Jr.: The closed conformation of a highly flexible protein: the structure of E. coli adenylate kinase with bound AMP and AMPPNP. (1994) Proteins 19:183-198.

　　(62) Russell, M. E., Utans, U., Wallace, A. F., Liang, P., Karnovsky, M. J., Wyner, L. R., Yamshita, Y. and Chi, T.: Upregulation of Gal/GalNAc macrophage lectin in chronically rejecting rat cardiac allografts with arteriosclerosis. (1994) J. Clin. Invest., 94:722-730.

　　(63) Utans, U., Liang, P., Wyner, L. R., Karnovsky, M. J. and Russell, M. E.: Chronic cardiac rejection: Identification of five upregulated genes in transplanted hearts by differential mRNA display. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:6463-6467.

　　(64) Sager, R., Anisowicz, A., Neveu, M., Liang, P. and Sotiropoulou, G.: Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene. (1993) FASEB J. 7:964-970.

　　(65) Liang, P., Averboukh, L. and Pardee, A. B.: Distribution and cloning of eukayotic mRNAs by means of differential display: refinements and optimization.(1993) Nucleic Acids Res. 21:3269-3275.

　　(66) Liang, P. Averboukh, L., Keyomarsi, K., Sager, R. and Pardee, A.B.: Differential display and cloning of mRNAs from human breast cancer vs. mammary epithelial cells. (1992) Cancer Research 52:6966-6968.

　　(67) Liang, P. and Pardee, A. B.: Differential display of eukaryotic mRNA by means of the polymerase chain reaction. (1992) Science 257:967-971.

　　(68) Liang, P., Phillips Jr., G. N. and Glaser, M.: Assignment of nucleotide binding sites and the mechanism of substrate inhibition of Escherichia coli adenylate kinase. (1991) Proteins 9:28-36.

　　(69) Liang, P. and Glaser, M.: Efficient cloning of a mutant adenylate-kinase-encoding gene from Escherichia coli. (1990) Gene 80:21-28.

　　(70) Althoff, S., Zambrowicx, B., Liang, P., Glaser, M. and Phillips Jr., G. N.: Crystalization and preliminary X-ray analysis of Escherichia coli adenylate kinase. (1988) J. Mol. Biol. 199:665-666.

　　(71) Liang, P. and Glaser, M.: Rapid recovery and Identification of a temperature- sensitive adenylate kinase gene from Escherichia coli. (1988) J. Cell Biol. 107:306a.

　　编著：

　　(1)Liang, P. and Pardee, A.B.: 《Differential display Methods and Protocols》. Methods in Molecular Biology，Vol. 85, Humana Press. 1979

　　(2)Liang, P.: 《Current progress in differential display methods and applications》. Methods -- A Companion to Methods in Enzymology, Academic Press, Vol. 16. 1998

　　(3)Liang, P. Meade, JD and Pardee, A.B.:《Differential Display Methods and Protocols》.2nd Edition. Methods in Molecular Biology, Vol. 317, Humana Press. 2005.

　　获奖：

　　1997 获一年一度的北美华人生物学家年会颁发的“科技发明大奖”

　　1998 获二年一度的德国分子生物学和生物化学协会颁发的“分子分析大奖”

　　2009 中组部2009年第一批“千人计划”入选者

　　2009 四川省第一批“百人计划”入选者

　　2009 国务院侨办“重点华侨华人创业团队”获得者

　　2009 国务院侨办颁布的第二届百名华侨华人专业人士“杰出创业奖”

　　专利：

　　(A)基因差异显示 (Differential Display) 技术发明(四项美国专利)

　　1993-11-16，Arthur Pardee,Liang Peng;Identifying, isolating and cloning messenger RNAs;USA;5, 262, 311

　　1997-2-4，Liang Peng，Arthur Pardee;Methods of differential display of exposed mRNA by RT/PCR;USA;5, 599, 672

　　1997-9-9，Arthur Pardee,Liang Peng;Methods of Comparing Levels or Amount of mRNAs;USA;5, 665,547

　　1999-8-12，Arthur Pardee,Liang Peng;System for Comparing level or amounts of mRNA;USA;5,965,409

　　(B)蛋白质三聚体化(Trimer-Tag)技术发明(三项美国专利)

　　2007-9-11，Liang Peng;Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins;USA;7,268,116

　　2010-2-23，Liang Peng;Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins;USA;7,666,837

　　2010-4-6，Liang Peng;Methods for Blocking TNF-alpha Activity in Mammals with Trimeric Soluble TNF Receptors;USA;7,691,815

　　(C)白介素24(IL-24)和白介素24受体(IL-24 receptors)发现(二项美国专利)

　　2005-6-7，Liang Peng;Human Mob-5 (IL-24) receptors and uses thereof;USA;6,902,930

　　2007-8-2，Liang Peng;Human Mob-5 (IL-24) receptors and uses thereof;USA;7,276,237

　　(D)p53靶基因Killin 发现(一项美国专利)

　　2009-8-8，Liang Peng, Yongjig Cho;Tumor suppressor Killin;USA;7,576,191