南京农业大学植物保护学院研究生导师：吴益东

　　吴益东 老师个人简介：

　　吴益东，教授，博士生导师。1994年获得南京农业大学昆虫专业博士学位，2001年晋升教授。先后在英国Rothamsted研究所、法国CIRAD和澳大利亚墨尔本大学遗传系进行2年合作研究。长期从事农业害虫抗药性研究，主持和承担国家自然科学基金、863、转基因生物品种培育重大科研专项、行业科技专项等课题15项。曾获教育部科技进步奖二等奖1项(1999，第1完成人)、教育部自然科学奖二等奖1项(2006，第2完成人)、国家科学技术进步奖二等奖1项(2010，第3完成人)。先后获得教育部首届高校青年教师奖(1999)、江苏省第六届青年科技奖(2000)、江苏省"333高层次人才培养工程"首批中青年科技领军人才(2007)、“新世纪百千万人才工程”国家级人选(2009)。

　　研究方向：

　　昆虫分子毒理，害虫抗药性，Bt作物抗性监测与治理

　　联系方式：

　　Tel: 025-84395261, e-mail: wyd@njau.edu.cn

　　主持的主要科研项目：

　　* 棉铃虫Cry1Ac抗性显性遗传品系的抗性机理及适合度，国家自然科学基金，2011-2013.

　　* Bt毒素Cry1Ac与棉铃虫中肠受体钙粘蛋白和氨肽酶的互作，国家自然科学基金，2009-2011.

　　* 棉铃虫对Bt棉花抗性频率监测与风险管理,转基因生物新品种培育重大专项，2011-2015.

　　* 小菜蛾抗药性监测与机理，公益性行业科技项目，2011-2015.

　　* 棉铃虫对Bt毒素抗性的高通量分子检测技术，863项目，2007-2010.

　　* 杀虫药剂诱导害虫再猖獗的机制，973项目子课题骨干，2006-2010.

　　* 棉铃虫田间种群钙粘蛋白基因变异与对Bt毒素抗性的关系，国家自然科学基金，2006-2008.

　　* 用RNA干涉技术研究棉铃虫细胞色素P450基因与抗药性的关系，国家自然科学基金，2005-2007.

　　* 小规模棉花生产体系棉铃虫的可持续控制，国际合作项目(CFC/ICAC/14)，2000-2005.

　　* BT棉在中国的农业和环境适合性评估，欧盟第五框架国际合作项目，2001-2005.

　　* 棉铃虫细胞色素P450同工酶的纯化及抗性基因检测，国家自然科学基金项目，1998-2000.

　　* 棉铃虫细胞色素P450与抗药性的关系，国家自然科学基金项目，1995-1997.

　　发表的主要论文 (*通讯作者)

　　Haonan Zhang, Wei Yin, Jing Zhao, Lin Jin, Yihua Yang, Shuwen Wu, Bruce E. Tabashnik, Yidong Wu*. 2011. Early Warning of Cotton Bollworm Resistance Associated with Intensive Planting of Bt Cotton in China. PLoS ONE 6(8): e22874.

　　Shuwen Wu, Yihua Yang, Guorui Yuan, Peter M. Campbell, Mark G. Teese, Robyn J. Russell, John G. Oakeshott and Yidong Wu*. 2011. Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera. Insect Biochemistry and Molecular Biology 41(1):14-21.

　　Zhenyu Wang, Haifei Yan, Yihua Yang and Yidong Wu*. 2010. Biotype and insecticide resistance status of the whitefly Bemisia tabaci from China. Pest Management Science.66(12):1360-1366.

　　Guorui Yuan, Weiyue Gao, Yihua Yang, Yidong Wu*. 2010. Molecular cloning, genomic structure and genetic mapping of two Rdl-orthologous genes of GABA receptors in the diamondback moth, Plutella xylostella. Achives of Insect Biochemistry and Physiology.74(2):81-90.

　　Youjing Gong, Chongli Wang, Yihua Yang, Shuwen Wu, Yidong Wu*. 2010. Characterization of resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella from China. Journal of Invertebrate Pathology. 104(2):90-96.

　　Xingliang Wang, Xiangyong Li, Aidong Shen, Yidong Wu*. 2010. Baseline susceptibility of the diamondback moth (Lepidoptera: Plutellidae) to chlorantraniliprole in China. Journal of Economic Entomology. 103(3):843-848.

　　Xin Pu, Yihua Yang, Shuwen Wu, Yidong Wu*. 2010. Characterization of abamectin resistance in a field-evolved multi-resistant population of Plutella xylostella. Pest Management Science. 66(4):371-378.

　　Jing Zhao, Lin Jin, Yihua Yang, Yidong Wu*. 2010. Diverse cadherin mutations conferring resistance to Bacillus thuringiensis toxin Cry1Ac in Helicoverpa armigera. Insect Biochemistry and Molecular Biology. 40(2):113-118.

　　Cun-Zheng Zhang, Zhi-Yong Zhang, Xian-Jin Liu, Wayne Jiang, Yi-Dong Wu*. 2010. Dissipation and environmental fate of herbicide H-9201 in carrot plantings under field conditions. Food Chemistry. 119:874-879.

　　Zhenyu Wang, Mingde Yao, Yidong Wu*. 2009. Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B biotype Bemisia tabaci. Pest Management Science. 65(11):1189-1194.

　　Yidong Wu*,Jean-Michel Vassal,Monica Royer,Isabelle Pieretti. 2009. A single linkage group confers dominant resistance to Bacillus thuringiensis δ-endotoxin Cry1Ac in Helicoverpa armigera. Journal of Applied Entomology.133(5):375-380.

　　Yihua Yang,Yajun Yang,Weiyue Gao,Jingjie Guo,Yanhong Wu,Yidong Wu*. 2009. Introgression of a disrupted cadherin gene enables susceptible Helicoverpa armigera to obtain resistance to Bacillus thuringiensis toxin Cry1Ac. Bulletin of Entomological Research.99(2):175-181.

　　Yihua Yang,Lina Yue,Song Chen,Yidong Wu*. 2008. Functional expression of Helicoverpa armigera CYP9A12 and CYP9A14 in Saccharomyces cerevisiae. Pesticide Biochemistry and Physiology.92(2):101-105.

　　Xinjun Xu and Yidong Wu*. 2008. Disruption of Ha_BtR alters binding of Bacillus thuringiensis δ-endotoxin Cry1Ac to midgut BBMVs of Helicoverpa armigera. Journal of Invertebrate Pathology. 97(1):27-32.

　　Yajun Yang, Haiyan Chen, Yidong Wu*, Yihua Yang, and Shuwen Wu. 2007. Mutated cadherin alleles from a field population of Helicoverpa armigera confer resistance to Bacillus thuringiensis toxin Cry1Ac. Applied and Environmental Microbiology.71(21):6939-6944.

　　Lihua Wang and Yidong Wu*. 2007. Cross-resistance and biochemical mechanisms of abamectin resistance in the B-type Bemisia tabaci. Journal of Applied Entomology. 131(2):98-103.

　　Yihua Yang, Song Chen, Shuwen Wu, Lina Yue, Yidong Wu*. 2006. Constitutive overexpression of multiple cytochrome P450 genes associated with pyrethroid resistance in Helicoverpa armigera. Journal of Economic Entomology. 99(5):1784-1789.

　　Yajun Yang, Haiyan Chen, Shuwen Wu, Yihua Yang, Xinjun Xu, Yidong Wu*. 2006. Identification and molecular detection of a deletion mutation responsible for a truncated cadherin of Helicoverpa armigera. Insect Biochemistry and Molecular Biology.36(9):735-740.

　　Ageng Li, Yihua Yang, Shuwen Wu, Chao Li, Yidong Wu*. 2006. Investigation of resistance mechanisms to fipronil in the diamondback moth (Lepidoptera: Yponomeutidae). Journal of Economic Entomology. 99(3):914-919.

　　Xinjun Xu, Liangying Yu, Yidong Wu*. 2005. Disruption of a cadherin gene associated with resistance to Cry1Ac δ-endotoxin of Bacillus thuringiensis in Helicoverpa armigera. Applied and Environmental Microbiology. 71(2):948-954.

　　Song Chen, Yihua Yang, Yidong Wu*. 2005. Correlation between fenvalerate resistance and cytochrome P450 mediated O-demethylation activity in Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Economic Entomology. 98(3):943-946.

　　Yang E., Yang Y., Wu S., Wu Y.*. 2005. Relative contribution of detoxifying enzymes to pyrethroid resistance in a resistant strain of Helicoverpa armigera. Journal of Applied Entomology. 129(9):521-525.

　　Yang Y., Wu Y.*, Chen S., Devine G.J., Denholm I., Jewess P., Moores G.D. 2004. The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia. Insect Biochemistry and Molecular Biology. 34(8):763-773.